A Soluble Cytochrome P-450 Functional in Methylene Hydroxylation
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چکیده
منابع مشابه
Regulation of Cytochrome P-450-supported 11P-Hydroxylation
It has been observed that bovine adrenocortical cells in culture fail to synthesize cortisol, while synthesizing deoxycortisol and other precursor steroids. The reason for this loss of activity of 11b-hydroxylase (cytochrome P-450118) was investigated. Treatment of cultures with 30 PM cortisol reduced the half-life of llb-hydroxylase activity from 40 h to 9 h. The concentration of cortisol caus...
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We have utilized 11beta-hydroxylase activity and visible absorption spectrophotometry to detect possible complex formation among adrenodoxin reductase, adrenodoxin, and cytochrome P-450(11)beta. At low ionic strength, a 1:1 complex between adrenodoxin reductase and adrenodoxin occurs but does not support maximal rates of 11beta hydroxylation; at least 1 additional molecule of adrenodoxin in exc...
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A cytochrome P-450 catalysing 25-hydroxylation of vitamin D3 was purified from liver mitochondria of untreated rabbits. The enzyme fraction contained 9 nmol of cytochrome P-450/mg of protein and showed only one protein band with an apparent Mr of 52,000 upon SDS/polyacrylamide-gel electrophoresis. The preparation showed a single protein spot with an apparent isoelectric point of 7.8 and an Mr o...
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The oxidation of norbornane by a reconstituted liver cytochrome P-450 system affords exoand endo-2-norborneol in a ratio of 3.4:1. The ratio ofese products was found to be 0.76:l when exo!exo,exo,exo-2,3,5,6-tetradueteronorbornane --was oxidized. Analysis of the mass spectra of the products from the deuterated hydrocarbon showed that 25% of the exo-norborneol contained four deuterium atoms wher...
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In previous publications [English, Hughes and Wolf (1994) J. Biol. Chem. 269, 26836-26841; English, Hughes and Wolf (1996) Biochem. J. 316, 279-283], we have demonstrated that peroxisome proliferators and non-steroidal anti-inflammatory drugs are inducers of the cytochrome P-450BM-3 gene in Bacillus megaterium ATCC14581. Their mechanism of action involves binding to and subsequent displacement ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)93343-0